They were arrested after a rally in Bulawayo where Mrs Mugabe spoke last Saturday, the state-owned Herald newspaper reports.
They have now been released on bail and have not yet commented on the charges.
The booing came amid a bitter battle between rival factions of the governing Zanu-PF party to succeed President Robert Mugabe, aged 93.
Mrs Mugabe leads one faction, while former Vice-President Emmerson Mnangagwa leads the other. He was sacked two days after the heckling and has since fled to neighbouring South Africa.
The four accused are said to be supporters of Mr Mnangagwa.
Meanwhile, an American satirist arrested last week for allegedly referring to President Mugabe as "a goblin" has been freed on bail due to what the judge called "an absence of facts".
SOURCE:AZANIA POST
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